TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidaDescription or relevant characteristicsbReference(s)
Strains
    Enterococcus faecalis
        DMC83006BClinical isolate, Vmr Gmr Smr Emr Lvr Hly+ Bac+This study, 3, 28
        WBH27862Clinical isolate, Vmr Gmr Smr Emr Lvr Hly+ Bac+This study, 3, 28
        SF830VsVms derivative of DMC83006B, Gmr Smr Emr Lvr Hly+ Bac+This study
        SFV1(DMC83006B X JH2-2), Rfr Far Vmr EmrThis study
        SFG1(SF830Vs X JH2-2), Rfr Far Gmr Smr Emr Bac+This study
        JH2-2Rfr Far19
        OG1RFRfr Far9
        OG1SSSmr Spr15
        OG1XSmr17
        OG1X/pAM373Smr, responder to pheromone cAM3737
        OGIX/pAD1Smr, responder to pheromone cAD117, 30
        OG1-10/pAMβ1Smr Emr6, 9
        OG1X/pIP501Smr Emr Cmr33
    Enterococcus faecium
        BM4105-RFRfr Far24
        BM4105-RF/pMG1Rfr Far Gmr31
    Staphylococcus aureus
        DMC82991AClinical isolate, MerThis study, 3, 28
        DMC83006AClinical isolate, Mer Vmr (MIC = 1,024μ g/ml)This study, 3, 28
        879R4RFRfr Far4
        RN4220/pGO1NARSA strain NRS10629
        SK5428SK982/pSK4111
Plasmids
    pAM830Tra+ Vmr Emr, 45 kbThis study
    pAM831Tra+ Gmr Smr Emr Bac+, 95 kbThis study
    pAM829Tra+ Gmr Kmr, 47 kbThis study
    pAM373Tra+, responds to pheromone cAM3734
    pAD1Tra+, responds to pheromone cAD130
    pCF10Tra+, responds to pheromone cCF108
    pPD1Tra+, responds to pheromone cPD134
    pAMβ1Tra+ Emr6
    pIP501Tra+ Emr Cmr21
    pMG1Tra+ Gmr18
    pGO1Tra+ Gmr Kmr Tmr29
    pSK41Tra+ Gmr Kmr12
  • a All clinical isolates are from the same patient. DMC83006B was isolated from a foot ulcer on 21 June 2002; WBH27862 was isolated from a catheter tip on 14 June 2002; DMC82991A was isolated from the nares on 21 June 2002; and DMC83006A was isolated from a foot ulcer on 21 June 2002.

  • b Cm, chloramphenicol; Em, erythromycin; Fa, fusidic acid; Gm, gentamicin; Km, kanamycin; Lv, levofloxacin; Me, methicillin; Rf, rifampicin; Sm, streptomycin; Sp, spectinomycin; Tm, trimethoprim; Vm, vancomycin; Bac, bacteriocin; Hly, Hemolysin; Tra, transfer; Tra+, self transfer for pAM831 is unproven.