TABLE 4.

ppGpp level in Fusr strains

fusA alleleRelative ppGpp levelc
BasalaInducedb
Wild type1.01.0
fusA1-10.91.3
fusA1-21.4ND
fusA1-71.71.1
fusA1-81.0ND
fusA1-111.2ND
fusA1-141.00.7
fusA1-151.30.6
fusA10.30.3
  • a The basal level of the wild type is 15 pmol of ppGpp/OD460 of exponentially growing cells.

  • b Induced levels are measured as the percent GTP converted into ppGpp 90 s after induction by the addition of α-methylglucoside. The value for the wild type is 29%. ND, not determined.

  • c All results are the arithmetic means of the results from at least three independent experiments. Our detection level in measurements of basal levels of ppGpp was 1 pmol/OD460, and the variation between experiments is approximately ±2 pmol/OD460.