TABLE 3.

Transfer of resistance

DonorRecipientSelectionaFrequencybAppearance of nonselected markers in transconjugantsc
% (No.) Vmr% (No.) Emr% (No.) Gmr
E. faecalis DMC83006BE. faecalis JH2-2Vm1.2 × 10−3*100 (216/216)0 (0/216)
Em1.3 × 10−3100 (250/250)*0 (0/250)
Gm4.0 × 10−77 (7/106)100 (106/106)*
E. faecalis WBH27862E. faecalis JH2-2Vm1.5 × 10−3*100 (230/230)0 (0/230)
Em1.8 × 10−3100 (234/234)*0 (0/234)
Gm8.6 × 10−76 (3/52)100 (52/52)*
E. faecalis SF830VsE. faecalis JH2-2Em5.4 × 10−7NA*100 (52/52)
Gm3.3 × 10−7NA100 (44/44)*
E. faecalis SFV1E. faecalis OG1XVm2.1 × 10−4*100 (217/217)NA
Em3.6 × 10−4100 (261/261)*NA
E. faecalis SFG1E. faecalis OG1SSGm<1.0 × 10−8NANA*
E. faecalis DMC83006BE. faecium BM4105-RFVm1.0 × 10−2*100 (164/164)0 (0/164)
S. aureus DMC83006AE. faecalis OG1RFVm<8.1 × 10−8*NANA
  • a Selection for transconjugants involved antibiotics for the recipient markers, as well as one of the following as indicated: Vm, vancomycin (50 μg/ml); Em, erythromycin (10μ g/ml); Gm, gentamicin (100μ g/ml).

  • b Transconjugants per donor. Transfer frequency represents the mean average of three overnight filter matings, except in the case of the S. aureus mating for which transfer frequency is based on a four-hour filter mating.

  • c *, selected marker; NA, not applicable. Parenthetical values are number resistant/number total.