TABLE 1.

Efficacy of antibiotics against internalized CD E. coli HM605 in J774-A1 macrophages at Cmax

Antibiotic (n)aDose (mg [route])bCmax(μg/ml)cReferenceMBC (μg/ml)dMIC (μg/ml)% Intracellular HM605 killing at Cmax (mean ± SEM)e
Ampicillin (9)500 (p.o.)3.223>100>25611.1 ± 5.4 (12.3 ± 7.8)
Azithromycin (3)500 (p.o.)0.495341.0 ± 10.5 (NA)
Ciprofloxacin (9)400 (i.v.)4610.30.00699.5 ± 0.2 (99.5 ± 0.3)
Clarithromycin (6)500 (p.o.)33231662.1 ± 5.6 (73.4 ± 3.2)
Rifampin (6)600 (p.o.)10.5483685.1 ± 6.6 (94.4 ± 1.0)
Sulfamethoxazole (6)800 (p.o.)40-6051NA>102461.3 ± 7.0 (65.3 ± 5.4)
Tetracycline (6)250 (p.o.)1.7441262.8 ± 6.1 (55.9 ± 4.4)
Trimethoprim (6)160 (p.o.)2.367100>3256.3 ± 3.4 (64.3 ± 8.4)
  • a n, number of experiments.

  • b p.o., orally; i.v., intravenously.

  • c Concentrations corresponding to the peak serum levels of the respective antibiotics following administration of conventional doses in humans based on published literature. The efficacy of various antibiotics on killing of internalized CD E. coli HM605 within J774-A1 macrophages at Cmax values is listed.

  • d Ampicillin caused 99.8% killing at the highest concentration tested (100 μg/ml). NA, no available data.

  • e Significant differences from antibiotic-free survival rates are indicated as follows: italics, P < 0.05; boldface, P < 0.001 (ANOVA). Values in parentheses represent data corrected for macrophage numbers at the end of incubation, as estimated by total cell protein assay (all, n = 3). NA, no available data.