TABLE 1.

Oligonucleotides used in this study

PrimerSequencea
D15′-(C/A)G(A/T)CC(A/T)GT(A/T)TT(T/C)GC(A/T)TGGGT-3′
D25′-AC(A/T)AC(A/T)AT(A/T)GC(A/G)TC(A/G)TC(A/T)AC-3′
AP15′-CATACGTTATGCCGTTACAGCTTC-3′
AP25′-CCATGAGAAACTGATTGCAGCTC-3′
AP35′-GTAGATCAGGCTCAAGCTGGTG-3′
AP155′-GCATGCCATGGGTATGATGTCGGCTAAGCTTTG-3′
AP165′-TCCGCTCGAGTTATTGCTTTTTAAGTTCAGCAC-3′
Ori15′-GCCACATGTGATCGTAGAAATATCTATGA-3′
Ori25′-GCCACATGTCGGATTTTAACATTTTGCG-3′
AP215′-GCGATAAAGTCATTGTTGATGGTG-3′
AP8r5′-CAGCATAGAGCACGCCAGAGAAG-3′
AP295′-CTTTCTTTGGTGGTACAACAGGTG-3′
AP7r5′-CGCAAGTGCAGACACCGCAATG-3′
S15′-TCCCAAGCTTGGCTAACCAAATCCAGTC-3′
S2r5′-TCCCAAGCTTACTGTCCAAACCTAGTGAG-3′
C15′-CAGCTCTAGATTTACGGACTGCTACGC-3′
C2r5′-CAGCTCTAGAGTAAGCCTTGTTGTGCAGC-3′
AACf5′-TACTGCAGTTGACACAGTGCCGTTGATCGTGCTATG-3′
AACr5′-ATTCTAGACTCATGAGCTCAGCCAATCGACTG-3′
  • a The restriction sites introduced into the oligonucleotides are underlined.