TABLE 2.

Plasmids and primers

Plasmid or primerSequenceDescriptionaReference or source
Plasmids
    pΔmecAEmr Tcr20
    pRN5543Cmr13
    pCN36Tcr11
Primers
    I1FGTTCCAGACGAAAAAGCACCAGAmplifies across the junction when SCCmec is excised34
    I1RCATTTTATGAGTCTCGCAAATTGTCAGAmplifies across the junction when SCCmec is excised34
    MRSAexrevCCTTTTGTATAATATATTCACATCACCAmplifies across the junction when SCCmec is excisedThis study
    WK1RCCGTAATTTACTATATTTAGTTGCAmplifies the excised, circular SCCmec elementThis study
    N315attRforGATCCTCGAGGAAATTCATTCGCATCAAACCTTTGATACAmplifies the excised, circular SCCmec elementThis study
    mecAFCTCATATAGCTCATCATACACTTTACCDetects mecAThis study
    mecARCACTTATTTTAATAGTTGTAGTTGTCGGDetects mecAThis study
    unirevGCACAGTGGGAATTAATCGAAGCLong-range PCR primerThis study
    252revCCACTATTTAACTGACTTGATATACCLong-range PCR primerThis study
    Map1FGGAAGATCTGATTGCTTAACTGCMaps SCCmec deletionThis study
    Map1RCTCTCTTGGTCGTCAGACTGATGGMaps SCCmec deletionThis study
    Map2FGCATGCTGCTTGCCTTAGGMaps SCCmec deletionThis study
    Map2RCACACAGCCAAAGCAATCAGCMaps SCCmec deletionThis study
    Map3FCCATTTGGCAGTTCTAAAAATCCGMaps SCCmec deletionThis study
    Map3RCGTAATACATTCGGTCATTGGGAAGCMaps SCCmec deletionThis study
    Map4FGGTTTCATGTTTGTGCTTCAGGMaps SCCmec deletionThis study
    Map4RCACGATACAAATCAAAAAAAGGTTGGMaps SCCmec deletionThis study
    Map6FCGTATCCTTTACAGGATATTTTGCMaps SCCmec deletionThis study
    Map6RCTGCATATTCTTGAATTTAAAAAGGMaps SCCmec deletionThis study
    Map7FGTTTCAGACTTTAGCGAGGAATGGMaps SCCmec deletionThis study
    Map7RCTATGTTGTATTTATCTTCGATAATGGMaps SCCmec deletionThis study
    Map8FGTGTTGCATTTGGTAGCCMaps SCCmec deletionThis study
    Map8RCGATGAGTTAAGAGCACGTATCMaps SCCmec deletionThis study
    Map9FCCGTTCGTTATAAATACTGCCMaps SCCmec deletionThis study
    Map9RCATGGAAAGTACATATAAAAAAAGAGGMaps SCCmec deletionThis study
  • a Cm, chloramphenicol; Em, erythromycin; Tc, tetracycline; r, resistant.