TABLE 1.

Correlation of the presence of point mutation G2576T in the 23S rRNA genes with the level of linezolid resistance in S. aureus

StrainNo. of passagesLinezolid MICb (mg/liter)Nucleotide at position 2576a in the 23S rRNA gene copies
rrn 1rrn 2rrn 3rrn 4rrn 5
T9911GGGGG
T188721c4GGTGG
T188822c8GGTGT
T190034c32GGTTT
T191044c64GTTTT
T201954c128TTTTT
T2019/5050d64TGTTT
  • a Escherichia coli numbering.

  • b MICs were determined by the Etest method (AB Biodisk, Solna, Sweden), using Columbia sheep blood agar (Heipha) for growth of the bacteria. MICs are given as the medians of the results of three experiments.

  • c Result represents passages of strain T991 under linezolid selective pressure.

  • d Result represents passages of T2019 in the absence of antimicrobial selective pressure.