TABLE 1.

Primers used for PCR and pyrosequencing

PrimerPositionaUseSequencebTarget species
23SV_univF_19261926-1948PCRTAAGGTAGCGAAATTCCTTGTCGUniversal primer; all species
Bio_23SV_univR_22592241-2259PCRBio-CGACCGCCCCAGTCAAACTcUniversal primer; all species
23SV_gpos_seq2038-2057PyrosequencingGGTTACCCGCGACAGGACGGGram-positive bacteria
23SV_gpos_seq2044-2057PyrosequencingCCGCGGCAAGACGGGram-negative bacteria
23SV_Hinf_seq2044-2057PyrosequencingCCGCGGCTAGACGGH. influenzae
23SV_myco_seq2041-2057PyrosequencingTACGYGCGGCGGACGAMycobacteria

  • ↵a Escherichia coli numbering.

  • ↵b Y = T/C.

  • ↵c Bio, biotin.