TABLE 7.

BM2 chemosensitizes S. cerevisiae constructs and fungal pathogens to azoles

Drug tested and speciesStrain (hyperexpressed protein)Concn used for chemosensitization/MICFold chemosensitization by BM2 (for FLC or ITC MIC)
BM2 (μM)FLC or ITC (μg · ml−1)
FLC
    S. cerevisiaeT481.25/2.55/204
AD/PDR5−1.25/2.50.5/12
AD/PDR5+ (Pdr5p)2.5/580/6007.5
AD1-8ua0.75/31/22
AD/PDR5 (Pdr5p)a1.75/3320/408
AD/CaCDR1 (CaCdr1p)a1.5/280/3204
AD/MDR1 (Mdr1p)1.5/23.75/6016
AD/ERG11 (Erg11p)1.5/20.5/24
AD/CaCDR2 (CaCdr2p)2/320/804
AD/CgCDR1-1B (CgCdr1p)a1.75/340/3208
    C. albicansATCC 1026110/200.25/>64>256
FR25/202/>32>16
KB20/4010/>160>16
    C. glabrataCBS1385/1020/>80>4
85082110/4080/1602
850905/402.5/>40>16
    C. tropicalisIF006181/31.25/>160128
    C. kruseiB239910/2020/>80>4
IFO 001110/2020/>80>4
89.10210/2040/>80>2
    C. dubliniensisCD365/200.3/>20>60
CD432.5/201.25/>20>16
    C. parapsilosis90.49310/402/84
42540/40>32/>32Not detected
ITC
    S. cerevisiaeAD1-8u1.5/30.2/0.84
AD/PDR51.75/310/>40>4
AD/CDR11.75/320/>40>2
AD/CgCDR1-1B1.75/420/>40>2
    C. glabrataCBS1385/100.2/>0.8>4
    C. kruseiB239910/200.05/>0.8>16
IFO 001110/200.05/0.816
89.10210/200.05/>0.8>16
  • a Incubated for 72 h instead of 48 h to allow full development of resistance phenotype.