TABLE 4.

Relative quantitations of total blaSHV and blaSHV in cis with the different promoters and PFGE

IsolateDDSTMIC (μg/ml)aMean copy number (range)Pulsotype
ATMCAZCTXblaSHVpr::IS26-blaSHVpr-blaSHV
B20.030.130.030.396 (0.356-0.440)Nilb0.082 (0.075-0.090)D
K20.030.130.031.00 (0.929-1.076)0.897 (0.783-1.028)0.102 (0.089-0.117)A
M10.030.130.030.444 (0.360-0.548)Nil0.128 (0.102-0.161)C
K10.060.50.060.331 (0.302-0.363)Nil0.178 (0.150-0.211)B
J50.060.50.130.696 (0.627-0.773)0.417 (0.395-0.441)0.175 (0.163-0.188)A4
J30.130.50.130.289 (0.258-0.324)Nil0.197 (0.161-0.242)A2
L20.130.250.250.395 (0.303-0.515)Nil0.124 (0.101-1.53)A5
J40.2520.131.875 (1.657-2.122)1.986 (1.642-2.402)0.072 (0.053-0.96)A2
F2+0.250.50.50.785 (0.643-0.958)0.313 (0.294-0.332)0.099 (0.094-0.105)A
A1+0.5110.794 (0.716-0.879)0.433 (0.391-0.480)0.189 (0.160-0.224)A
E1+0.5110.664 (0.570-0.774)0.613 (0.503-0.746)0.197 (0.138-0.282)A6
B1+1110.655 (0.547-0.785)0.427 (0.384-0.475)0.150 (0.132-0.172)A5
F1+1110.707 (0.661-0.757)0.369 (0.307-0.445)0.084 (0.068-0.105)A
D1+2441.701 (1.556-1.860)1.721 (1.490-1.987)0.085 (0.069-0.104)A1
L1+323210.809 (0.689-0.949)0.319 (0.254-0.400)0.087 (0.079-0.096)A
J2+643221.692 (1.552-1.848)1.277 (1.064-1.533)0.060 (0.018-0.206)A5
C1+646440.835 (0.752-0.927)0.613 (0.508-0.739)0.133 (0.114-0.155)B
J1+643240.968 (0.793-1.182)1.32 (1.16-1.51)0.124 (0.092-0.167)A3
G1+128128162.955 (2.498-3.496)3.854 (3.418-4.347)0.158 (0.122-0.206)A
I1+128128164.616 (4.203-5.070)5.241 (4.863-5.648)0.093 (0.086-0.102)A
H1+1281281288.244 (7.628-8.909)8.111 (6.776-9.709)0.124 (0.102-0.150)A1
  • a ATM, aztreonam; CAZ, ceftazidime; CTX, cefotaxime.

  • b On occasion, amplimer appeared very late in the cycling protocol, i.e., at least seven cycles later than for the isolates with measurable pr::IS26-blaSHV. This was not reproducible (i.e., on most occasions the PCRs were entirely negative) and was probably due to contamination.