TABLE 5.

Effects of BM0 and BM2 on growth, viability, and Pma1p activity for S. cerevisiae and fungal pathogens

Species and strainMIC90/MFCa (μM)IC50 (μM) for Pma1p ATPaseConcn (μM)/time (h) to >98% cell deathb
BM0BM2BM0 (pH 7.0)BM2 (pH 7.0)BM0 (pH 7.0)BM2 (pH 7.0)
pH 7.0pH 7.3 (7.5)cpH 7.0pH 7.3 (7.5)c
S. cerevisiae
    T481.3s0.63s/2.5c5s0.63s/2.5c2.50.5100/17.520/17.5
    AD/PDR5+10c2.5c5c1.25cNDNDNDND
    AD/PDR5−10c1.3s/2.5c2.5s/10c0.63s/1.25cNDNDNDND
Fungal pathogen
    C. albicans ATCC 10261>2020s>105s/10c202100/2.040/2.0
    C. glabrata CBS138>20>1010s5s/10c102100/17.520/17.5
    C. krusei B2399>2020s>1010s61100/17.540/1.0
    C. tropicalis IF016820c10s/20c5s2.5s/5c50.625/0.55/0.5
    C. neoformans ATCC 9012110c5s/10c5c2.5s/10c31.525/0.510/2.0
  • a MIC90, minimum concentration at which there is >90% growth inhibition compared to no-peptide control (s, fungistatic); MFC, peptide concentration at which no CFU was recovered (c, fungicidal).

  • b Concentration of peptide that gives >98% cell death/time required to achieve this effect. ND, not determined.

  • c The pHs in parentheses are those used in tests with fungal pathogens other than S. cerevisiae. A single pH value indicates that the same pH was used for both S. cerevisiae and the other fungal pathogens.