TABLE 2.

Oligonucleotide primers used for amplification and sequencing of virulence-related loci in this study

ProductPrimerSequence (5′-3′)GenBank accession no.Position
clfA R domainclfAF1ATG GGA CAA CGA AGT AGC A Z18852 1867-1885
clfAR1GCT TCA TCT TCA GAA CCT G2997-3015
clfB R domainclfBF2GTT ATG GTG GTG GAA GTG CTG AJ224764 1613-1633
clfBR1CGC TCT TAT CTC CTG TTT CTG G2650-2671
fnbA D, W, and M regionsfnbF1TAG GAA CTG AAA ATG GTC AC J04151 2255-2274
fnbAR1GAA GCA ATC AGA AAA CAC TC3262-3281
fnbB D, W, and M regionsfnbF1TAG GAA CTG AAA ATG GTC AC X62992 2465-2484
fnbBR1GAG TAT GTA ATT ATT TCT TGG3417-3437
agrC 5′ regionagrCF1GAA TTA ACD CAA TTA CAC GA AF001783 685-704
agrCF1CAA TTT CTT CTT GAT TAC G AF001783 1366-1384
agrCF2CCA TTG AAA TCA CTC CTT CC X52543 1483-1502
agrCR2GAT AGA CCT AAA CCA CGA CC AF001783 1906-1925
RNAIII and P2-P3 interpromoterRNAIIIF1GAC CTT TTC CAA CAT TAG AC X52543 972-991
RNAIIIR1ACA CCA CTC TCC TCA CTG TC1752-1771
RNAIIIR2AGA TAC GTG GCA AAC TGG TC1800-1819