TABLE 2.

Kinetic PCR results

IsolateaPhenotypic databKinetic PCRc at codon:
DDSTMIC (μg/ml)238240
ATMCAZCTXCTΔCTCTΔCT
MutantWild typeMeanSDMutantWild typeMeanSD
B20.030.130.0322.016.0−4.91.2225.117.7−6.60.82
K20.030.130.0321.714.5−7.00.3323.813.1−10.40.55
M10.030.130.0324.118.2−6.80.7128.720.1−7.32.67
K10.060.500.0621.815.1−6.70.2724.117.8−5.90.52
J50.060.500.1324.616.1−8.01.6822.313.1−9.10.39
J30.130.500.1322.617.5−4.11.1621.014.4−6.50.78
L20.130.250.2526.417.2−8.31.3024.115.0−8.51.01
J40.252.000.1321.015.1−6.00.2521.311.7−9.30.31
F2+0.250.50.516.417.91.30.5024.713.6−10.60.75
A1+0.51115.216.71.40.3123.312.9−10.00.58
E1+0.51115.919.22.21.2324.813.7−9.71.39
B1+11116.317.81.30.2023.114.1−8.80.42
F1+11115.216.61.40.2822.013.3−9.00.76
D1+24414.217.02.90.7122.412.5−9.81.05
L1+3232115.316.41.50.4615.013.7−0.90.43
J2+6432215.916.00.20.3814.713.0−1.80.49
C1+6464416.116.0−0.70.4814.918.44.10.68
J1+6432416.616.4−0.20.2215.514.0−2.51.48
G1+1281281612.915.22.70.3711.714.52.80.19
I1+1281281613.015.42.30.5911.714.73.40.28
H1+12812812811.614.32.70.4710.614.83.90.45
  • a Designations are as in reference 6.

  • b The DDST and MIC data are from reference 17. The highest concentration tested when the isolates were characterized (17) was 128 μg/ml, so some MICs may be higher than this. ATM, aztreonam; CAZ, ceftazidime; CTX, cefotaxime.

  • c Shown are data from single replicates of CT measurements from each isolate and the mean ΔCT and associated standard deviation from all replicates.