TABLE 1.

MICs of the SM and MC switch variants

Growth condition and antifungal treatmentSM MIC (μg/ml)MC MIC (μg/ml)
Flu MIC AM3 103a22
Flu MIC AM3 103 + glucose11
Flu MIC AM3 10544
Flu MIC AM3 105 + glucose44
AMB MIC AM3 1030.250.25
AMB MIC AM3 103 + glucose0.250.25
AMB MIC AM3 1050.250.125
AMB MIC AM3 105 + glucose0.250.125
Flu MIC RPMI 10344
Flu MIC RPMI 103 + glucose44
Flu MIC RPMI 10588
Flu MIC RPMI 105 + glucose88
AMB MIC RPMI 1030.250.25
AMB MIC RPMI 103 + glucose0.50.5
AMB MIC RPMI 1050.250.5
AMB MIC RPMI 105 + glucose0.50.5
1 h TK assay 1 log reduction after FlubNIcNIc
4 h TK assay 1 log reduction after Flub88
1 h TK assay 1 log reduction after AMB1.00.5
4 h TK assay 1 log reduction after AMB1.00.5
  • a MIC of Flu determined in medium AM3 with a starting inoculum of 103 per ml.

  • b Time-kill assay to determine the MIC of Flu that leads to a 1-log reduction of control CFU after 1 or 4 h of coincubation.

  • c NI, no inhibition by Flu after 1 h at concentrations up to 256 μg/ml.