TABLE 3.

Summary of kinetic properties and Ki values of the strains included in the study

OrganismStrainFKS1-HS1 sequenceaVmax (nmol/min)bKm (mM)bKi (ng/ml)b
ANFCSFMCF
C. parapsilosis 22019FLTLSLRDA0.52 ± 0.140.60 ± 0.16639.50 ± 10.6147.99 ± 0.72476.00 ± 2.83
C. parapsilosis H4FLTLSLRDA0.44 ± 0.160.45 ± 0.07368.50 ± 13.443.93 ± 2.22486.00 ± 7.07
C. parapsilosis H5FLTLSLRDA0.46 ± 0.040.59 ± 0.05431.67 ± 15.896.01 ± 0.71261.00 ± 11.31
C. orthopsilosis H10FLTLSLRDA0.33 ± 0.200.51 ± 0.07111.00 ± 5.664.29 ± 1.11177.50 ± 2.12
C. orthopsilosis 981224FLTLSLRDA0.40 ± 0.040.93 ± 0.1146.50 ± 12.027.40 ± 2.69248.00 ± 2.83
C. metapsilosis am-2006-0113FLTLSLRDA0.18 ± 0.070.22 ± 0.1341.40 ± 5.0976.90 ± 0.85266.00 ± 8.49
C. metapsilosis 960161FLTLSLRDA0.25 ± 0.200.20 ± 0.02129.00 ± 11.3180.45 ± 0.49382.50 ± 2.12
S. cerevisiae BY4742FLVLSLRDP2.65 ± 0.160.10 ± 0.0268.95 ± 12.51183.94 ± 35.27179.01 ± 8.08
S. cerevisiae BY4742-P649AFLVLSLRDA1.58 ± 0.130.13 ± 0.082374.0 ± 9.901215.00 ± 12.735706.50 ± 20.51
C. albicans Sc5314FLTLSLRDP6.72 ± 0.250.12 ± 0.011.33 ± 0.562.97 ± 1.5827.47 ± 1.82
C. albicans 90028FLTLSLRDP5.87 ± 0.200.09 ± 0.011.43 ± 0.140.95 ± 0.0523.53 ± 2.37
C. albicans 36082FLTLSLRDP4.71 ± 0.480.07 ± 0.010.63 ± 0.180.46 ± 0.0415.47 ± 2.05
C. albicans M122FLTLSLRDH1.95 ± 0.290.15 ± 0.01646.07 ± 26.72291.67 ± 50.162588.53 ± 32.25
C. glabrata 90030FLILSLRDP6.97 ± 0.570.13 ± 0.0116.15 ± 1.0618.95 ± 3.041.13 ± 0.18
C. glabrata T51916FLILSLRDT0.37 ± 0.060.05 ± 0.01712.00 ± 9.90194.50 ± 23.33510.00 ± 67.88
  • a FKS2-HS1 sequences are shown for C. glabrata strains. Substituted amino acids are in bold.

  • b Arithmetic means ± standard deviations (three repetitions).