TABLE 3

Kinetic parameters of nitrocefin hydrolysis by LdtMab2 and LdtMt2a

EnzymeMean Vmax (μM/min) ± SDMean Km (μM) ± SD
LdtMab2wt19.8 ± 2.03328.3 ± 65.5
LdtMab2Y331F9.7 ± 0.6155.7 ± 25.8
LdtMab2H333N12.9 ± 0.5147.5 ± 15.0
LdtMab2H349N18.8 ± 5.31,010 ± 395
LdtMab2C351A18.6 ± 5.81,022 ± 441
LdtMt2wt0.74 ± 0.0230.0 ± 2.9
LdtMt2H336N0.96 ± 0.03163.3 ± 14.4
LdtMt2H352N0.69 ± 0.02115.7 ± 11.1
LdtMt2C354A0.81 ± 0.02140.8 ± 6.9
  • a wt, wild type.