TABLE 1.

Cholesterol import in A. fumigatus cultured with different concentrations of human seruma

MeasurementImport levels at serum concn (%):
00.331030
Cell mass (mg/250 ml)237 ± 27286 ± 33454 ± 41545 ± 45587 ± 9
Total cholesterol in medium (mg)01.3513.545135
Cholesterol import (mg/250 ml)00.4 ± 0.19.0 ± 0.612.5 ± 2.713.9 ± 3.2
Cholesterol (μg/mg of cell)01.6 ± 0.619.9 ± 2.522.7 ± 3.223.8 ± 5.5
Cellular ester/free cholesterol3.4 ± 0.44.9 ± 0.25.4 ± 0.65.5 ± 0.5
Free cholesterol (μg/mg of cell)00.4 ± 0.23.4 ± 0.53.6 ± 0.83.6 ± 0.7
Cholesteryl ester (μg/mg of cell)01.2 ± 0.516.5 ± 2.019.1 ± 2.420.0 ± 4.8
Ergosterol (μg/mg of cell)2.1 ± 0.81.9 ± 0.52.3 ± 0.22.4 ± 0.13.0 ± 0.3
Cellular free/ester ergosterol16.4 ± 6.726.6 ± 8.7NDNDND
Free ergosterol (μg/mg of cell)2.0 ± 0.71.8 ± 0.42.3 ± 0.22.4 ± 0.13.0 ± 0.3
Ergosterol ester (μg/mg of cell)0.1 ± 0.00.1 ± 0.10.0 ± 0.00.0 ± 0.00.0 ± 0.0
Total free sterol (μg/mg of cell)2.0 ± 0.72.2 ± 0.65.7 ± 0.46.0 ± 0.86.6 ± 0.8
Cellular free cholesterol/free ergosterol0.0 ± 0.00.0 ± 0.01.5 ± 0.31.5 ± 0.41.2 ± 0.3
Total cellular cholesterol/ergosterol0.0 ± 0.01.8 ± 0.18.7 ± 1.79.6 ± 1.58.1 ± 2.4
  • a Results are from 250-ml cultures in 1-liter flasks. Sterols were quantitated by 1H NMR analysis of the Folch extract of lyophilized mycelia. Data represent the means ± standard deviations of three independent experiments. ND, not determined. The ratio of ester/free cholesterol in human serum was 3.3 ± 0.0 as measured with the same modified Folch extraction.