TABLE 1.

Plasmids used in this work

PlasmidRelevant characteristic(s)Source or reference
pMAC9.5-kb plasmid of A. baumannii (ATCC 19606T) involved in organic peroxide resistance 15
pTVICU5311-kb plasmid of A. baumannii TVICU53This work
pYMAb3RepM and Ori fragments of pMAC were subcloned into a pET28a-based vectorThis work
pOXA-58-3A fragment containing IS1008-ΔISAba3-blaOXA-58 was subcloned into an E. coli and A. baumannii shuttle vector, pYMAb3This work
pOXA-58-4A fragment containing ΔISAba3-blaOXA-58 was subcloned into an E. coli and A. baumannii shuttle vector, pYMAb3This work
pET28aBackbone of pYMAb3; KmrNovagen
pMAL-c2xRestriction with HindIII and treatment with alkaline phosphatase; used for cloning of a HindIII-generated fragment of pTVICU53New England Biolabs