TABLE 2.

Effects of serum or purified cholesterol on ITC-treated A. fumigatusa

TreatmentITC (μg/ml)Cell mass (mg)Ergosterol (μg/mg)Cholesterol (μg/mg)
Control0.00185 ± 202.9 ± 0.3
0.0178 ± 8b*1.9 ± 0.1b*
0.0333 ± 2b*0.6 ± 0.0b*
0.120 ± 0b*
Serum (3%)0.00460 ± 34b*2.9 ± 0.124.2 ± 1.9
0.03260 ± 4c*2.2 ± 0.1c**35.8 ± 0.7
0.1299 ± 3c*1.2 ± 0.1c*68.5 ± 3.2
Cholesterol (54 μg/ml)0.00211 ± 113.1 ± 0.161.1 ± 2.1
0.01202 ± 15c*2.7 ± 0.0c*60.5 ± 2.8
0.03111 ± 4c*2.3 ± 0.0c*105.5 ± 3.5
0.126 ± 1c*NDND
  • a Cell mass represents dried cells from 250-ml cultures. Sterol levels were determined from 1H NMR analysis of the NSL of lyophilized cells. ND, not determined. These data represent the means ± standard deviations of three independent experiments. *, P < 0.001; **, P < 0.05.

  • b Comparison with control (0% serum and 0% ITC; n = 6).

  • c Comparison with same dose of drug-treated cells with no serum or cholesterol.