TABLE 4.

RT-PCR analysis of acrA expression

GeneStrainCtaRelative expression vs that of rrsEbOverexpression ratioc
acrAG85830.93.32e-648.5
G149225.61.61e-4
rrsEG85812.7
G149213.0
  • a Ct (critical threshold cycle) numbers represent an average for duplicate samples with a final RNA concentration of 40 ng/ml.

  • b Relative expression is calculated as 2−ΔΔT, where ΔΔT = Cttarget − Ctreference.The target is acrA, and the reference is rrsE.

  • c Ratio between relative expression level of the target gene in G1492 versus that in G858.