TABLE 1.

E. coli strains used in this study

StrainDescriptionReference
3-AG100MKXaacrAB-overexpressing gyrA mutant derived from AG100MKX (AG100 marA::Kmr)2
DC14AG100 ΔacrAB::Kmr1
1-DC14PSgyrA mutant derived from DC14PS after selection with ofloxacin1
2-DC14PSbacrEF-overexpressing mutant derived from 1-DC14PS after selection with ofloxacin1
HS414Wild-type W31108
HS276HS414 ΔacrEF ΔacrAB ΔyhiUV ΔacrD ΔyegMNO::Kmr8
  • a Enhanced expression of acrAB without expression of acrEF was shown by reverse transcription-PCR and was associated with diminished intracellular accumulation of ofloxacin in the absence of CCCP (2).

  • b Enhanced expression of acrEF was shown by reverse transcription-PCR and was associated with diminished intracellular accumulation of ofloxacin in the absence of CCCP (2).