TABLE 2.

Primers used for PCR amplification and sequencing

Target geneSequenceAmplicom size (bp)
pbp1a (SAG0298, gbs0288)f1 5′-CGGAATTCATGGGATTTATTATCTTAGCTA-3′2,209
r1 5′-ACGTCGACTTAATTACCGTTAGGTACTGTA-3′
f1b 5′-ACACCAAAGAAGAAATTCTTAC-3′
f2 5′-TAAAGCAAAAATCTACTTATCC-3′
    Sequencing primersf2b 5′-GTAGTGAGAAAATGGCAGCGGC-3′
f3 5′-GCCTACATGATGACGGATATGC-3′
f3b 5′-CAAAATTCTGGACAGTCAAGTC-3′
r2 5′-TCCAATCTGCACTGTATCCGCC-3′
r3 5′-TAGCTGCTTTAGTACCAGTACC-3′
r4 5′-CAGCGGCTTCAAGTGCTCTGAC-3′
r5 5′-TGACTTTACCATTAGTCGCATC-3′
r6 5′-TTTTATCTTGATACATCTGCTG-3′
pbp1b (SAG0159, gbs0155)f1 5′-CGGAATTCATGTTTAAAGGTAATAAGAAGT-3′2,314
r1 5′-ACGTCGACTTATCGTTTTCCACCCAAAGTA-3′
f1b 5′-GGTTTGGAGAGAGTAGCGG-3′
f2 5′-CTATTGTATATTCTCCTTATAC-3′
    Sequencing primersf2b 5′-GTATACTATTAAAACTACTATC-3′
f3 5′-TGATGTAAAAAACTATATGGAG-3′
f3b 5′-CCTGTCCGTGTCTTTTCGAAAG-3′
r2 5′-GTGTAGAAAGCATCAACCAAAC-3′
r3 5′-GAGCAACTGACGTATCAATACC-3′
r4 5′-GATCAATAGCAATTCCGTAAGG-3′
r5 5′-TTTTTAAATCATGCTCTGAAAC-3′
r6 5′-GTAAACCTGCAAGGAAAGCTGC-3′
pbp2a (SAG2066, gbs2020)f1 5′-CGGGATCCATGAAATTATTTGATAAGTTTA-3′2,338
r1 5′-ACGTCGACCTATCTAAAGTAGTCCTTTAGA-3′
f1b 5′-TGCTCTAAAAACAACCACCACC-3′
    Sequencing primersf2 5′-ATCTTAATAACTCTTATTTTGG-3′
f2b 5′-GGTATGAAAAATAGATTAGCAG-3′
f3 5′-TCCTGCTGTTTATACTTTAGAC-3′
f3b 5′-ACTCGAATTGAGACAGCTAATG-3′
r2 5′-CTGTCAAATAATGGTGTTTATC-3′
r3 5′-ATGAGCGCGATGCATTATACCG-3′
r4 5′-GTTCTTTATCTATTGACCATCC-3′
r5 5′-TATAGCCATTATTGACAATATC-3′
r6 5′-TCAAATTAGCAGCACTGGTTCC-3′
pbp2b (SAG0765, gbs0785)f1 5′-CGGAATTCATGTTGAATCGTAAAAAAAGGT-3′2,062
r1 5′-ACGTCGACTTATTGTCCTGTGAACTGTGAA-3′
f1b 5′-TTCATCTCAGTCTATCAAAGAG-3′
    Sequencing primersf2 5′-CTATTTCTACAGAAAAGGCAGG-3′
f2b 5′-AGAAAGTATCTTGAAACAATAC-3′
f3 5′-CAACTCTAATGGAATCGTTCGG-3′
f3b 5′-TGGACAAACAGTTTCTACCTAC-3′
r2 5′-CTATCTTATTTAGTGTTTTAGG-3′
r3 5′-GATAGCCTCGATCAGTTAAAGC-3′
r4 5′-CATGATCATTTTTCAGACCAGC-3′
r5 5′-CTCGGTCATTCAGTGAATAGCC-3′
r6 5′-TAGCGCTCACTGGAACTGCAGC-3′
pbp2x (SAG0687, gbs0277)f1 5′-CGGAATTCGTGACTTTTTTTAAAAAGCTAA-3′2,275
r1 5′-ACGTCGACTTAATCTCCTATTGTAATTTTG-3′
f1b 5′-AACTATACGACAGCTACAGGTC-3′
    Sequencing primersf2 5′-GTAGTGGGAATGTTCTTTTAGG-3′
f2b 5′-TCTAAGCATTTTAACTCTACTG-3′
f3 5′-AAGAAGCAGCTAGTAAAACACG-3′
f3b 5′-GAAAATCCAGGTCATGTAGCGG-3′
r2 5′-GAACCAGATTACGACGTAATTC-3′
r3 5′-CAGATTTTACTGCAACTGATTG-3′
r4 5′-ATGAGCTCATAGCGATAGTTAC-3′
r5 5′-TTGCAGAGGCTAGAGTCATTAC-3′
r6 5′-CCGCCCTACGTTCTGTTGTTGC-3′
r7 5′-AAGACAATCCTGAACCTGAACTTCC-3′
r8 5′-TATCTGTACCAACGATGATGAC-3′