TABLE 3.

Binding of [3H]telavancin and [3H]vancomycin to MRSA cells and cellular fractions

DrugdKAA (mM)Amt (ng) in total cultureaAmt (ng) (%)b in cellular fraction:
PGProtoplast
Telavancin0306.8 ± 1.5 (23)23 ± 2 (77)
0.42.80.8 ± 0.07 (29)2 ± 0.1 (71)
Vancomycin01312 ± 2 (95)0.6 ± 0.1 (5)
0.40.320.3 ± 0.06 (94)0.02 ± 0.007 (6)
  • a Amount of compound recovered in the combined peptidoglycan and protoplast fractions derived from 75 ml of cell culture incubated with 375 ng of compound.

  • b Amount of compound recovered in the indicated fraction ± standard deviation of the mean from three separate experiments, with the percentage of total bound compound recovered in the indicated fraction noted in parentheses. PG, peptidoglycan.