TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidDescriptionSource or reference
Strains
    TOP10 E. coli cloning strainInvitrogen
    INV110 dam and dcm methylase-deficient E. coliInvitrogen
    G340Clinical isolateThis study
    G595Clinical isolateThis study
    G815Clinical isolateThis study
    GC7535G340 insertion mutant, IS903φkan inserted in ramAThis study
    GC7736G595 transformed with pCLL3442This study
    GC7737GC7535 transformed with pCLL3442This study
Plasmids
    pCR2.1-TOPOPCR cloning vectorInvitrogen
    pUCGmpUC19 derivative containing gentamicin resistance cassette 18
    pVJT128Plasmid carrier vector for IS903φkan transposon 20
    pCLL3441pCR2.1-TOPO with cloned ramA geneThis study
    pCLL3442pCLL3441 with cloned gentamicin cassetteThis study