TABLE 1.

Bacterial plasmids and strains used in this study

Plasmid or strainDescriptionSource or reference
Plasmids
    pGEMT-EasyPCR cloning vector, AmprPromega
    pCMERpGEMT-Easy containing full-length cmeR fragment, AmprThis study
    pCMERCpCMER with chloramphenicol resistance cassette inserted in cmeR, Ampr CmrThis study
    pMW10E. coli-C. jejuni shuttle vector with promoterless E. coli lacZ gene, Kanr52
    pIT81pMW10 derivative with the cmeABC promoter of wild-type C. jejuni 81-176 inserted upstream of lacZThis study
    pIT3epMW10 derivative with the cmeA promoter of C. jejuni CR3e inserted upstream of lacZThis study
    pRK2013IncP Tra RK2+ ΔrepRK2 repE1+, Kanr6
    pQE-30Expression vector, AmprQiagen
    pQE-CmeRpQE-30 derivative expressing full-length recombinant CmeR proteinThis study
Strains
    C. jejuni
        NCTC 11168Wild type; genome sequence known35
        81-176Wild type; isolated from a human4
        CR3e81-176 derivative; spontaneous fluoroquinolone-resistant mutant obtained after stepwise selection with ciprofloxacinThis study
        9B681-176 derivative; cmeB::kan24
        JL10681-176 derivative; cmeC::kan25
        JL10781-176 derivative; cmeR::cmThis study
        JL10881-176 containing pMW10This study
        JL109JL107 containing pMW10This study
        JL11081-176 containing pIT81This study
        JL111JL107 containing pIT81This study
        JL11281-176 containing pIT3eThis study
    E. coli
        JM109endA1 recA1 gyrA96 thi hsdR17 (rk, mk+) relA1 supE44 Δ(lac-proAB) [F′ traD36 proAB lacIqZΔM15]Promega
        DH5αF φ80lacM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17 (rk, mk+) phoA supE44 thi-1 gyrA96 relA1 λInvitrogen