TABLE 1.

MICs and MFCsa

IsolateAmphotericin BItraconazoleVoriconazole
MIC/MFC (μg/ml)EC50 (μg/ml) ± 95% CIHill slope ± 95% CIMIC/MFC (μg/ml)EC50 (μg/ml) ± 95% CIHill slope ± 95% CIMIC/MFC (μg/ml)EC50 (μg/ml) ± 95% CIHill slope ± 95% CI
A. fumigatus 2930.25/20.10 ± 0.06−3.84 ± 2.100.25/20.30 ± 0.10−2.40 ± 1.000.25/0.500.18 ± 0.06−3.07 ± 1.10
A. fumigatus 99-20750.25/0.50.12 ± 0.08−3.25 ± 1.200.5/160.85 ± 0.46−0.56 ± 0.450.25/10.10 ± 0.02−2.66 ± 0.60
A. fumigatus 5830.25/0.50.10 ± 0.01−4.27 ± 2.1032/≥3212.3 ± 2.00−0.56 ± 0.310.25/20.37 ± 0.07−1.20 ± 0.23
A. terreus 09251/20.53 ± 0.10−5.33 ± 3.201/80.83 ± 0.12−3.37 ± 1.820.25/40.68 ± 0.19−2.15 ± 0.62
A. terreus 09321/40.36 ± 0.06−2.65 ± 2.100.5/80.24 ± 0.05−3.13 ± 2.000.25/40.10 ± 0.04−1.43 ± 0.60
F. solani 00116/≥32NCbNC≥32/≥32NCNC16/≥32NCNC
F. solani 0021/20.27 ± 0.12−1.62 ± 0.638/≥32NCNC4/323.30 ± 1.30−0.87 ± 0.50
F. oxysporum 0014/80.55 ± 0.35−0.86 ± 0.66≥32/≥32NCNC4/≥323.12 ± 1.70−0.81 ± 0.67
F. oxysporum 0022/40.41 ± 0.26−0.80 ± 0.308/≥32NCNC2/≥320.53 ± 0.27−0.54 ± 0.32
S. apiospermum4/>320.97 ± 0.35−0.57 ± 0.522/≥320.64 ± 0.35−1.17 ± 0.610.25/160.27 ± 0.025−1.21 ± 0.80
S. prolificans1/40.65 ± 0.32−0.77 ± 0.22≥32/≥32NCNC16/≥32NCNC
  • a MICs and MFCs were determined in triplicate with standardized methods and are reported as median values (16). Pharmacodynamics data were generated from 24-h XTT colorimetric assays performed in triplicate. The EC50 was calculated by fitting inhibition data to a four-parameter logistic model (Hill equation). All experiments were performed in triplicate. CI, confidence interval.

  • b NC, not calculable due to minimal antifungal activity.