TABLE 4.

RT-PCR analysis of ramA expression

GeneStrainCTaRelative expression level versus rrsEbOverexpression ratioc
ramA G34026.52.09e − 336.7
G59531.35.69e − 5
rrsE G34017.6
G59517.2
  • a CT values represent averages for duplicate samples with a final RNA concentration of 1 ng/ml.

  • b Relative expression is calculated as 2−ΔΔT. ΔΔT = CT(target) − CT(reference), where the target is ramA and the reference is rrsE.

  • c The overexpression ratio is the ratio between the relative expression level of the target gene in G340 versus that in G595.