TABLE 1.

Strains and plasmids used in this study

Strain or plasmidDescriptionReference
S. aureus strains
    Mu3Clinical MRSA isolate with heterogeneous resistance to vancomycin15
    N315Clinical MRSA isolate17
    RN4220Restriction deficient derivative of 8325-416
Plasmids
    pALC2073pSK236 containing the tet(R) gene and the tetracycline-inducible xyl/tetO promoter region cloned into the PstI and SmaI sites2
    pCLL3432pALC2073 with tet(M) cloned into the EcoRI site in the sense directionThis study
    pCLL3433pALC2073 with tet(M) cloned into the EcoRI site in the antisense directionThis study
    pCLL3434pALC2073 with mepR cloned into the SacI site in the sense directionThis study
    pCLL3435pALC2073 with mepR cloned into the SacI site in the antisense directionThis study
    pCLL3436pALC2073 with mepA cloned into the EcoRI site in the sense directionThis study
    pCLL3437pALC2073 with mepA cloned into the EcoRI site in the antisense directionThis study