TABLE 5.

Identities between ORFs of the novel SCCmec II variant element of Irish MRSA isolate AR13.1/3330.2 and the conventional SCCmec IVb and II elements

SCCmec element and ORFLocationaHomology to ORFs of SCCmec IVbbHomology to ORFs of SCCmec IIb
ORF% HomologycGene productORF% HomologycGene product
IIE01210-1145 (complement)CM00183.7Hypothetical protein
IIE021123-1893 (complement)CM002100Hypothetical protein
IIE032010-3440M00199.6Hypothetical protein
IIE043560-5350M00299.7Conserved hypothetical proteinN03180.3Conserved hypothetical protein
IIE05 (ccrA2)5584-6933M00499.1Cassette chromosome recombi- nase A2N03496.7Cassette chromosome recombi- nase A2
IIE06 (ccrB2)6955-8583M00597.8Cassette chromosome recombi- nase B2N03798Cassette chromosome recombi- nase B2
IIE079101-9451M00688.9Conserved hypothetical proteinN041100Conserved hypothetical protein
IIE089538-9849M00789.4Conserved hypothetical proteinN042100Conserved hypothetical protein
IIE099867-10373M00891.7Conserved hypothetical proteinN04399.4Conserved hypothetical protein
IIE1010394-10711dN044100Pseudogene
IIE11 (tnpA)10830-11915N045100Transposase A
IIE12 (tnpB)11912-13804N046100Transposase B
IIE13 (tnpC)13811-14188N047100Transposase C
IIE14 (spc)14339-15121N048100O-Nucleotidyltransferase
IIE15 (ermA)15247-15978 (complement)CN026100rRNA adenine N-6-methyltrans- ferase
IIE16 (tnp IS1182)16499-18103
IIE17 (ΔmecI)18207-18459 (complement)CN03667.7Methicillin resistance protein MecI
IIE18 (mecR1)18459-20216 (complement)CM00355.6Truncated signal transducer protein MecR1CN037100Signal transducer protein MecRI
IIE19 (mecA)20316-22322M010100Penicillin binding protein 2aN058100Penicillin binding protein 2a
IIE2022368-22796 (complement)CM004100Conserved hypothetical proteinCN038100Conserved hypothetical protein
IIE21 (ugpQ)22893-23636CM006100Glycerophosphoryldiester phosphodiesteraseN039100Glycerophosphoryldiester phos- phodiesterase
IIE22 (tnp IS431mec)23901-24575M011100Transposase for IS431mecN062100Transposase for IS431mec
IIE2324607-24846 (complement)CM007100Conserved hypothetical proteinCN049100Conserved hypothetical protein
IIE2425261-26556 (complement)CM00840.7Truncated conserved hypo- thetical proteinCN050100Truncated conserved hypothe- tical protein
  • a The nucleotide positions were determined based on the sequences for SCCmec IIE deposited in the GenBank database under accession number AJ8101208.

  • b ORFs and gene products from SCCmec II and SCCmec IVb, based on nucleotide sequences in the GenBank database, accession number D86934 and AB063173, respectively (17, 25).

  • c Based on amino acid identity.

  • d —, no significant homology.