TABLE 2.

Nucleotide sequences of blaTEM-coding regions and their promoters

Nucleotidea (amino acidb) positionNucleotide (amino acid)c in blaTEM-coding regions and their promoters
blaTEM-1Ad (P3)blaTEM-93 (Pa/Pb)blaTEM-1Fe (P4)blaTEM-1Bf (P3)blaTEM-25g (?)blaTEM-4h (Pa/Pb)blaTEM-94 (Pa/Pb)blaTEM-48i (Pa/Pb)blaTEM-49i (P3)blaTEM-47i (Pa/Pb)blaTEM-68j (Pa/Pb)blaTEM-29k (Pa/Pb)blaTEM-85 (Pa/Pb)blaTEM-86 (Pa/Pb)
32CT?TTTTTTTT
162GT?
175AG?GG
226CTTT
263 (21)C (Leu)T (Phe)T (Phe)T (Phe)T (Phe)T (Phe)T (Phe)T (Phe)
346AGGGGGGGGG
436CTTTTTTTTTTTT
512 (104)G (Glu)A (Lys)A (Lys)
604GT
682TCCCCCCCCCCC
692 (164)C (Arg)A (Ser)A (Ser)
693 (164)G (Arg)A (His)
747 (182)T (Met)C (Thr)C (Thr)
911 (237)G (Ala)A (Thr)
914 (238)G (Gly)A (Ser)A (Ser)A (Ser)A (Ser)A (Ser)A (Ser)A (Ser)A (Ser)
917 (240)G (Glu)A (Lys)A (Lys)A (Lys)A (Lys)A (Lys)A (Lys)A (Lys)
925GAAAAAAAAAAA
990 (265)C (Thr)T (Met)T (Met)T (Met)T (Met)T (Met)T (Met)T (Met)T (Met)T (Met)
998 (268)A (Ser)G (Gly)
1020 (275)G (Arg)T (Leu)
  • a Nucleotide positions are numbered according to Sutcliffe (36).

  • b Amino acid positions are numbered according to Ambler et al. (1). The numbers are indicated only for positions in which substitutions were observed.

  • c Only mutational changes with respect to the TEM-1 β-lactamase and/or blaTEM-1A gene sequences are listed.

  • d Reference 36.

  • e Reference 27.

  • f Reference 21.

  • g Reference 10.

  • h Reference 34 and this work.

  • i Reference 19.

  • j Reference 15.

  • k Reference 2 and this work.