TABLE 3.

Antimicrobial susceptibilities of the clinical isolates; their E. coli A15 transconjugants; and the TEM-85-, TEM-86-, TEM-93-, and TEM-94-producing E. coli DH5α transformants

Strain no. and ESBL variantMIC (μg/ml)a
AMPPIPTZPCEFCTXCTX + CLACAZCAZ + CLAATMATM + CLAFOXIPMAMKGEN
K. pneumoniae SU2949/98 TEM-85>512>5121286420.551221280.5320.12525664
K. pneumoniae SU3220/98 TEM-85>512>51243210.0651222560.2540.125256128
K. pneumoniae SU3519/98 TEM-85>512>51286440.06>5124512140.125256256
E. coli SU3408/98 TEM-85>512256≤0.5161≤0.03640.25320.0640.25128128
K. pneumoniae SU2946/98 TEM-86>512128425640.061281160.12540.12525664
E. coli SU2947/98 TEM-2925625616320.250.0640.1250.250.0620.2521
K. pneumoniae GD3658/98 TEM-47>512>51232>51280.2512811280.12580.125132
K. pneumoniae GD3661/98 TEM-47>512>5124>512320.2525615120.25320.125132
K. pneumoniae GD3670/98 TEM-47>512>51216>512160.2512811280.125320.125164
E. coli GD3667/98 TEM-47>512256≤0.52568≤0.03160.12516≤0.0320.1252128
E. coli GD3657/98 TEM-94>5122561>512320.06320.25160.0680.12564256
E. coli GD3668/98 TEM-94>512>5122>5121281641640.53211632
E. coli KR 3445/98 TEM-93>512256251240.06640.25640.0680.12510.5
E. coli KR 3447/98 TEM-93>512512251280.06640.5640.0680.12510.5
K. pneumoniae CZ 9455/99 TEM-4 (+CTX-M-3)b>512>51232>5122562642640.51280.125>256>256
K. pneumoniae CZ 9459/99 TEM-4 (+CTX-M-3)b>512>51264>5122562642640.5640.25>256>256
K. oxytoca BB 1753/98>5125121>512640.0640.12540.125640.25>256>256
TEM-4 (+CTX-M-3)
R+ (E. coli SU3408/98) TEM-85>512128≤0.580.25≤0.03640.25160.0640.5116
R+ (K. pneumoniae GD3661/98, GD3670/98, and GD3667/98) TEM-47>51264-128≤0.51281-2≤0.0316-320.1258-16≤0.031-20.1250.5-116-32
R+ (E. coli GD3657/98 and GD3668/ 98) TEM-94>512128≤0.51284≤0.038-160.1252≤0.0310.125432
R+ (E. coli KR3445/98) TEM-93>512256≤0.52562≤0.03320.12516≤0.0320.250.50.5
R+ (K. pneumoniae CZ9455/99 and CZ9459/99) TEM-4>51264-1281-264-1284-8≤0.0340.062-4≤0.0320.12516-3264
R+ (K. oxytoca BB1753/98) TEM-4>5126411284≤0.0340.1252≤0.0320.125>256>256
E. coli DH5α(pGBT-85) TEM-85>512128≤0.5162≤0.032560.251280.2520.25NDND
E. coli DH5α(pGBT-86) TEM-86>51232≤0.52564≤0.03640.2580.12520.25NDND
E. coli DH5α(pGBT-93) TEM-93>512128≤0.51282≤0.0380.068≤0.0320.125NDND
E. coli DH5α(pGBT-94) TEM-94>512128≤0.51284≤0.0380.064≤0.0320.125NDND
E. coli A15 R2≤0.5≤0.52≤0.03≤0.030.1250.1250.06≤0.0310.1250.50.25
E. coli DH5α2≤0.5≤0.52≤0.03≤0.030.1250.060.06≤0.0320.125NDND
E. coli ATCC 2592242280.06≤0.030.250.1250.060.0640.12510.5
  • a Abbreviations: AMP, ampicillin; PIP, piperacillin; TZP, piperacillin with tazobactam; CEF, cephalothin; CTX, cefotaxime; CLA, clavulanate; CAZ, ceftazidime; ATM, aztreonam; FOX, cefoxitin; IPM, imipenem; AMK, amikacin; GEN, gentamicin; ND, not determined; R+, transconjugants; R, E. coli strain without plasmids, used as a recipient in mating.

  • b Some of the MICs for these isolates were published previously (4).