TABLE 2.

Effect of ribosome-targeted antibiotics and NRIs on tRNA protection at the decoding site in 16S rRNA

InhibitorProtectiona:
With tRNA and poly(U)Without tRNA and poly(U)Binding domain in 30S
A1492/A1493 sitesA1413A1408A1492/A1493 sitesA1413A1408
30S inhibitors
    Apramycin++++NA
    Gentamicin+++++H44
    Kanamycin+++H44
    Neomycin+++++H44
    Paromomycin+++++H44
    Streptomycin+++H44
    Hygromycin B+/−+/−H44
    KasugamycinH24
    SpectinomycinH34
    TetracyclineH34
    PuromycinNA
50S inhibitors
    Linezolid
    Chloramphenicol
    Fusidic acid
    Kirromycin
    Sparsomycin
NRIs
    A-72310++
    A-692345++
    A-424902++
    A-358816++
    A-362505++
    A-438301
    A-548501
  • a “+” denotes increased tRNA protection on the specified 16S rRNA sites with >70% band intensity reduction at 100 μM drug compared to that observed in the absence of the drug; “+/−” denotes a weak effect with 30 to 70% band intensity reduction at 100 μM; “−” denotes a lack of protection effect with <30% band intensity reduction at 100 μM. NA, not available or known.