TABLE 1.

Strains and plasmids used in this study

Strain or plasmidRelevant genotype or propertiesaSource or reference
PAO1wild type13
PAO325Δ(mexAB-oprM)b nfxB Δ(mexCD-oprJ) Δ(mexJKL) Δ(mexXY) OpmH+4
PAO327Δ(mexAB-oprM) nfxB Δ(mexCD-oprJ) Δ(mexXY) mexL MexJ+K+4
PAO346PAO327 with ΔopmD302cThis study
PAO351PAO327 with ΔopmM81This study
PAO353PAO327 with ΔaprF153This study
PAO355PAO327 with ΔopmL133This study
PAO357PAO327 with ΔopmF123This study
PAO359PAO327 with ΔopmG151This study
PAO361PAO327 with ΔopmA21This study
PAO363PAO327 with ΔopmB282This study
PAO365PAO327 with ΔopmE209This study
PAO367PAO327 with ΔopmH362This study
PAO369PAO327 with ΔopmK53This study
PAO371PAO327 with ΔopmI184This study
PAO373PAO327 with ΔopmJ177This study
PAO375PAO327 with opmN::ISlacZ/hah-TcThis study
PAO377PAO327 with ΔopmQ395This study
PAO386PAO325 with ΔopmH362This study
PAO397PAO386 with Δ(mexEF-oprN)This study
8802Tetr; PAO1 with opmN::ISlacZ/hah-Tc14
KG4510Δ(nfxB-mexCD-oprJ) mexR ΔoprM Δ(mexXY)21
pBSP II KS(−)/SK(−)Apr; broad-host-range cloning vectors23
pCR2.1Apr; TA cloning vector for PCR fragmentsNovagen
pVLT35Spr/Smr; broad-host-range cloning vector6
pPS1150Apr; pBSP II SK(−) with 6,945-bp NotI mexJKL fragment4
pPS1266Apr; pCR2.1 with 1,673-bp opmH PCR fragmentThis study
pPS1313Spr/Smr; pVLT35 with 1,664-bp PstI-HindIII opmH fragment from pPS1266This study
pPS1319Apr; pCR2.1 with 1,563-bp EcoRV-HindIII oprM PCR fragmentThis study
pPS1369Apr; pCR2.1 SpeI(blunt)+XhoI with 4,369-bp SgfI(blunt)-XhoI mexJK fragment from pPS1150This study
pPS1370Apr; pBSP II KS(−) carrying a 4,394 bp BamHI-XbaI mexJK fragment from pPS1369This study
pPS1371Apr; pPS1370 BamHI(blunt)+HindIII with 1,530-bp EcoRV-HindIII oprM fragment from pPS1319This study
pPS1372Apr; pPS1370 BamHI(blunt)+HindIII with 1,659-bp PstI(blunt)-HindIII opmH fragment from pPS1266This study
pPS1373Apr; pPS1371 HindIII(blunt)+ClaI with 1,665-bp PstI(blunt)-ClaI opmH fragment from pPS1389This study
pPS1389Apr; pBSP II KS(−) with 1,664-bp PstI-HindIII opmH fragment from pPS1266This study
pPS1424Apr; pBSP II KS(−)XhoI(blunt)+HindIII with 1,659-bp PstI(blunt)-HindIII opmH fragment from pPS1266This study
pPS1425Apr; pBSP II KS(−)XhoI(blunt)+HindIII with 1,530-bp EcoRV-HindIII oprM fragment from pPS1319This study
  • a Abbreviations: Apr, ampicillin resistance; FRT, Flp recombinase target; Gmr, gentamycin resistance; Smr/Spr, streptomycin/spectinomycin resistance; Tetr, tetracycline resistance.

  • b All deletions are unmarked but contain an FRT scar insertion; e.g., Δ(mexAB-oprM)::FRT.

  • c Allele numbers indicate the number of codons deleted from the respective genes.