TABLE 1.

Primer sequences used for amplification and sequencing of the hot spot regions within CgFKS1 and CgFKS2

PrimerSequence
FKS1
    HS1
        Forward5′-CCATTGGGTGGTCTGTTCACG
        Reverse5′-GATTGGGCAAAGAAAGAAATACGAC
        Sequencing5′-CTCAAACCTTCACTGCCTC
    HS2
        Forward5′-GGTATTTCAAAGGCTCAAAAGGG
        Reverse5′-ATGGAGAGAACAGCAGGGCG
        Sequencing5′-CGGTATGAATGCCCTATTACG
FKS2
    HS1
        Forward5′-GTGCTCAACATTTATCTCGTAGG
        Reverse5′-CAGAATAGTGTGGAGTCAAGACG
        Sequencing5′-GCTTCTCAGACTTTCACCG
    HS2
        Forward5′-CGTAGACCGTTTCTTGACTTC
        Reverse5′-CTTGCCAATGTGCCACTG
        Sequencing5′-TCTTGACTTTCTACTATGCG