TABLE 2.

Genetic regions and ORFs of plasmid pABIRa

FeaturePositionGene product (no. of amino acids)Properties and/or putative functionHomology (GenBank/EMBL accession no. match)
oriV1-200Origin of DNA replicationpAB02 (AY228470)
Repeat region217-312Imperfect 4-repeat iterons; control of DNA replication
repA357-1244295RepA AB; replicasepAB02 (AY228470)
ORF1321-1884188Unknown
ORF2179-2721180Hypothetical protein similar to COG 0790 FOG; TPR repeat, SEL1 subfamilypMAC (AY541806), pACICU1 (CP000864)
ORF2964-326098Putative inner membrane proteinpAB02 (AY228470), pAB2 (CP000523)
ORF3247-3561104Putative cytoplasmic proteinpAB02 (AY228470), pAB2 (CP000523)
ORF4455-4766103Unknown
ORF5235-5714153Unknownp2ABAYE (CU459138)
ORF6210-6581123UnknownpACICU1 (CP000864), p2ABAYE (CU459138), pAB2 (CP000523)
oriV7450-7650Origin of DNA replication
Repeat region7651-77605-repeat iterons; control of DNA replication
repAci17815-8150112Plasmid replication protein;
9238-9855205    truncated by ISAb125
ISAb1258160-9230322ISAb125/ISAb125 transposaseISAba125 (AY751533)
Tn539311279-13148611Tn5393 transposase disrupted by IS26 insertionSalmonella enterica serovar Paratyphi A (AM412236); uncultured bacterium pRSB105 (DQ839391)
IS2613329-13916195IS26 transposasepACICU1 (CP000864), uncultured bacterium pRSB105 (DQ839391)
IS1814349-15422320IS18/IS18 transposaseAcinetobacter sp. strain BM2716 (AF043676)
blaOXA-5815553-16395280Carbapenem-hydrolyzing oxacillinaseAY665723, pACICU1 (CP000864), EF102240
ISAba316433-17222145IS element ISAba3/ISAba3 transposaseAY665723, pACICU1 (CP000864), EF102240
trr17366-18193275Arac1 binding protein; arabinose operon control protein; transcriptional regulatorAY665723, EF102240
lysE18243-18848201Threonine efflux proteinAY665723, EF102240
Recombination point18843-18869Repeat region 1/Re27-1AY665723, EF102240
ORF18914-19243109Unknown
ORF19244-1952593Putative transcriptional regulatorGeobacter metallireducens GS-15 (CP000148) (73% identity)
ORF19605-20030141Unknown
Recombination point20062-20088Repeat region 2/Re27-2AY665723, EF102240
ORF20164-20484106UnknownEF102240
ORF20477-2074990Helix-turn-helix XRE family cdsEF102240
mel21242-22717491Macrolide efflux proteinEF102240, uncultured bacterium pRSB105 (DQ839391)
mph222773-23657294Macrolide 2′-phosphotransferase
Resolvase23847-24449200Resolvase
ORF25126-25533135Unknown
ORF25629-2587782Unknown
ORF28643-29032129UnknownAcinetobacter venetianus pAV1 (DQ278485)
  • a Sequences in the GenBank/EMBL database showing identity or high similarity are indicated. Microorganisms from which DNA sequences have been isolated are indicated when different from A. baumannii. COG, conserved domain in bacteria; cds, coding sequences.