TABLE 1.

Primers used in this study

Primer nameaSequence (5′ to 3′)TargetPurpose(s)b
PAampD1RTF1GTGGTGGTCTACAGTCTCGampD1R
PAampD1RTR1CGAAGAAGCTCTGGTAGCCampD1R
PAampDh2RT3FCTACACCTCCACCGATCTGampDh2R
PAampDh2RTRGATCTCGATGCCGATCGAGampDh2R
PAampDh3RTAFCAGCTATCGCACCACTACCampDh3R
PAampDh3RTB2RCGACCAGGTTGAAGATGCGampDh3R
SodBF1CTCGAAGAGATCGTCAAGAGsodBR
SodBR1CGGTAGTCGATGTAGTAGGCsodBR
PAampDFGACGATGCCTTGCTGTTCGampD1P, C, S
PAampDRseqGATCCTCGACCAGGCTCAGCampD1P, C, S
PAampDh2CFGCACCACCTTCGACGGTCCampDh2P, C, S
PAampDh2RCGAGCCTTTCGTCCAGGTCampDh2P, C, S
PAampDh3FGACCGCTGCGAAAGGCTCTGampDh3P, C, S
PAampDh3RGTGCGACGGCATTCATGGCampDh3P, C, S
PAampRCSeqFGAACAGCAGTGTGGAAGCGampR-ampC intergenic regionP, S
PAampRSeqR2CGAGAGCGAGATCGTTGCampR-ampC intergenic regionP, S
PAampRSeqIRCAGCAGAGCACCGTCAGCampR-ampC intergenic regionS
PAampRSeqIFGCGCTGGCGTTGTTCGAGampR-ampC intergenic regionS
ECamiDRTFGGTCAGCTCGCATTATCTGGamiDR
ECamiDRTRGAATCTGTGCCGGTTCAAACGamiDR
ECamiDFGCAGTATGCTGATGGTTAGCamiDP, C, S
ECamiDRGTCGTATTCGTTCCGTCCamiDP, C, S
  • a All primers with names beginning with PA and the SodBF1 and SodBR1 primers were designed using the PAO1 genome sequence (GenBank accession number NC_002516). Those with names beginning with EC were designed using the E. coli K-12 genome sequence (GenBank accession number NC_00913).

  • b R, RT-PCR; P, PCR; C, cloning; and S, sequencing.