TABLE 3.

Diversity of STs among E. faecium blood isolates with different antibiotic and virulence profiles

MLSTClonal complexPFGE typeAMP profileaNo. of isolatesAllelic profilebAFLP typeVIEP markerc
purKadkatpAddlgydgdhpstSesphyl
ST-18CC-17AREF-AR11171511C
ST-18CC-17AREF-DR41171511C−/+
ST-18CC-17AREF-JR11171511C
ST-18CC-17AREF-MR11171511C
ST-18CC-17AREF-CR11171511C+
ST-18CC-17ASEF-9S11171511C
ST-17CC-17AREF-PR21111111C+
ST-16CC-17AREF-TR11112111C++
ST-63CC-17AREF-WR221111111C+
ST-103CC-17AREF-WR1211114111C+
ST-4ASEF-31S13584111NT
ST-22ASEF-2S12123111B
ST-71ASEF-3S121153111A
ST-40ASEF-32S11661086810R
ST-74ASEF-15S1226221361427R
  • a AMP, ampicillin; R, resistant; S, susceptible.

  • b adk, gene coding for adenylate kinase; atpA, gene coding for ATP synthase, alpha subunit; ddl, gene coding for d-alanine:d-alanine ligase; gyd, gene coding for glyceraldehyde-3-phosphate dehydrogenase; gdh, gene coding for glucose 6-phosphate dehydrogenase; pstS, gene coding for phosphate ATP binding cassette transporter.

  • c Presence (+) or absence (−). Some isolates belonging to the same PFGE type showed variable presence of a given VIEP marker (−/+).