TABLE 2.

Strains and plasmids used in transposition experiments

Strain or plasmidRelevant characteristicsaSource or reference
E. coli
    DH1F supE44 recA1 endA1 gyrA96 (Nalr) thi-1 hsdR17 (rK mK+) relA120
    HB101F hsd20(rB mB) proA2 lacY1 recA13 rpsL20 (Strr) ara-14 galK2 xyl-5 mtl-1 supE44 λ2
    K802NhsdR hsdM+ gal met sup rpoB nalA (Nalr)25
Plasmids
    pOX38-NeorF derivative Tra+ Neor; 58.2 kb3
    pSU18-MluITra Mob Cmr; 2.3 kbThis study
    pAT783pSU18-MluIΩ22 kb MluI-AscI from pIP1204 Tra Mob Cmr Smr Sulr Tpr 4,6-ddsrThis study
    pBR322Tra Mob Apr Tcr; 4.4 kb22
    pIP1204IncL/M Tra+ Apr Cefr Smr Sur Tpr 4,6-ddsr; ca. 90 kb7
  • a Tra+, self-transferable; Tra, non-self-transferable, Mob, nonmobilizable; Apr, ampicillin resistance; Cefr, cefotaxime resistance; Cmr, chloramphenicol resistance; Nalr, nalidixic acid resistance; Neor, neomycin resistance; Smr, streptomycin-spectinomycin resistance; Strr, streptomycin resistance; Sur, sulfonamide resistance; Tcr, tetracycline resistance; Tpr, trimethoprim resistance; 4,6-ddsr, 4,6-disubstituted deoxystreptamine resistance.