TABLE 2.

Summary of in vitro properties of C. neoformans (1,3)β-glucan synthase activity

PropertyValue
Linear with time (min)0∼60
Linear with protein (μg)25∼240
Specific activity (nmol min−1 mg−1)0.045
Lysate activity after freeze/thaw (%)100
100% Lysate activity, stored at −80°C (weeks)>25
(1,3)β-glucan in product (%)>75
Km(app) (mM)0.21 ± 0.09
Vmax(app) (nmol min−1)0.01 ± 0.0007