TABLE 1.

Bacterial strains used in this study

Strain or plasmidDescriptionaSource or reference
P. aeruginosa
    K767PAO1 prototroph 29
    K1521K767 ΔmexCD-oprJ 46
    K1536K767 nfxB 18
    K1523K767 ΔmexB 18
    K1542K767 ΔmexB ΔmexXYThis study
    K2443K767 ΔalgUThis study
    K2895K1536 ΔalgUThis study
    K2896K1542 ΔmexCD-oprJThis study
    K2897K1542 ΔalgUThis study
    K2898K2896 ΔalgUThis study
    K2899K1542 carrying a PmexCD-oprJ -lacZb transcriptional fusion at the attB siteThis study
    K2900K1542 carrying a promoterless lacZ gene at the attB siteThis study
    K2901K2897 carrying a PmexCD-oprJ -lacZ transcriptional fusion at the attB siteThis study
    K2902K2897 carrying a promoterless lacZ gene at the attB siteThis study
    K2888PAO1 prototroph 26
    K2890K2888 mexD::mini-Tn5-luxCDABE 26
    K2889CHX-passaged K2888This study
    K2891CHX-passaged K2890This study
E. coli
    DH5αφ80dlacZΔM15 Δ(lacZYA-argF) endA1 recA1 2
    S17-1 thi pro hsdR recA Tra+ 43
Plasmids
    pK18MobSacBBroad-host-range gene replacement vector; sacB Kmr 43
    miniCTX-lacZIntegration vector with promoterless lacZ; oriT+ Tcr 4
    pFLP2Source of Flp recombinase; Apr/Cbr 19
    pMMB206 P. aeruginosa and E. coli shuttle cloning vector; Cmr 32
    pEX18TcGene-replacement vector; sacB Tcr 19
    pCSV05pEX18Tc::ΔmexXY 7
    pRSP05pK18MobSacB::ΔmexCD-oprJ 46
    pSF01pK18MobSacB::ΔalgUThis study
    pSF02pMMB206::algUThis study
    pAJC03miniCTX-PmexCD-oprJ-lacZbThis study
  • a Apr, ampicillin resistance; Cmr, chloramphenicol resistance; Cbr, carbenicillin resistance; Kmr, kanamycin resistance; Tcr, tetracycline resistance.

  • b The mexCD-oprJ promoter region upstream of a promoterless lacZ gene.