TABLE 1.

Tobramycin resistance mutations causing defective growth in LB broth under aerobic and/or anaerobic conditions and small-colony variants

Defect or variant typePAO1 ortholog(s) of mutant geneGene nameGene description
General growth defectPA0432 sahH S-Adenosyl-l-homocysteine hydrolase
PA1547Putative membrane protein
PA1548Putative cytochrome oxidase maturation protein, cbb3 type
PA1588 sucC Succcinyl-CoA synthetase beta subunit
PA4429Putative cytochrome c1 precursor
PA4430Putative cytochrome c reductase, iron-sulfur subunit
PA5070 tatC Sec-independent protein translocase TatC
Aerobic growth defectPA3818Inositol-1-monophosphatase
PA3975Possible phosphomethylpyrimidine kinase
Anaerobic growth defectPA1480 ccmF Cytochrome c-type biogenesis protein CcmF
PA2638-PA2648 nuo NADH dehydrogenase I
PA2960 pilZ Type IV fimbrial biogenesis protein PilZ
PA3233Putative signal transduction protein
PA4673Putative GTP-binding protein
PA4916Putative ADP-ribose pyrophosphatase
Small-colony variants (energyPA1480 ccmF Cytochrome c-type biogenesis protein CcmF
    metabolism mutations)PA1483 cycH Cytochrome c-type biogenesis protein
PA1552Putative cytochrome c oxidase, cbb3 type, subunit III
PA1553Putative cytochrome c oxidase, cbb3 type, subunit II
PA1554 ccoN Cytochrome oxidase subunit, cbb3 type
PA4429Putative cytochrome c1 precursor
PA4430Putative cytochrome b
PA4431Putative cytochrome c reductase, iron-sulfur subunit
PA5300 cycB Cytochrome c5