TABLE 3.

Features of the original insertion sequences detected in Tn5393d

Insertion sequenceFamilySize (bp)Inverted repeataPutative transposase
IS1012IS1788GGTGGTGTTTCAAAAAGTATGCTGA-738 bp-TCAGCATACTTTTTGAAACACCACC233 aab; closest relative: IS1 transposase of Salmonella enterica serovar Typhimurium (GenBank accession no. AAR05766 ; 28.5% amino acid identity)
IS1387aIS41,387TCATACGTATGTCTTTAG-1,350 bp-CTAAAGA(T)CATACGTATGA421 aa; closest relatives: putative transposase of IS1387b (62.4% amino acid identity) and putative transposase from marine psychrotrophic bacterium Mst37 (GenBank accession no. CAC84124 ; 33.3% amino acid identity)
IS1387bIS41,387TCATACGTATGACCTTAA-1,351 bp-TAAAGATCATACCTATGA423 aa; closest relatives: putative transposase of IS1387a (62.4% amino acid identity) and putative transposase of marine psychrotrophic bacterium Mst37 (GenBank accession no. CAC84124 ; 34.6% amino acid identity)
IS1066IS301,066CCTGAATTCAACACATAAGTGCAACGCT-1,010 bp-AGCGTTGCGCTTATTCTATGAATTCAGG320 aa; closest relative: putative transposase of an IS30 family insertion sequence form Aeromonas punctata (GenBank accession no. AAS66625 ; 45.1% amino acid identity)
  • a The nucleotide sequences of the inverted repeats; residues in parentheses are insertions which do not match in the other IR; underlined residues indicate mismatches between the two IRs.

  • b aa, amino acid.