TABLE 1.

Primers used for generation of the probes used for Southern blot experiments for screening the pFL424 library, and/or for PCR mapping of Tn5393da

Primer (sequence)TargetAnnealing temp (°C)b
BLAPER/f (5′-GGGACARTCSKATGAATGTCA) bla PER gene50
BLAPER/r (5′-GGGYSGCTTAGATAGTGCTGAT) bla PER gene50
IR5393/u (5′-TTGGCCARCGATATTCTCCGGTRAGATT)IRs of Tn539350 or 60
aphA6/f (5′-GGAAACAGCGTTTTAGAGCC) aphA6b gene50
aphA6/r (5′-GCTTCACGAGAGACACTGTA) aphA6b gene50
  • a The locations and orientations of the primers are shown in Fig. 1. The strategy adopted for PCR mapping is described in Materials and Methods.

  • b The annealing temperature used in the PCR experiments. Two values are present for primer IR5393/u: the highest temperature was used when the primer was used alone for long PCR amplification of the entire element; the lowest temperature was used when the primer was used in combination with either the aphA6/r or the BLAPER/f primer.