TABLE 2.

Sequences of primers designed for this study

PrimerSequence (5′→3′)aLocation and/or primer type
RA-1GCAGCCAAATCCCAACAATTAAGGarr-2
RA-2CGCCGCCATAAACGGCGACAGGarr-2, reverse primer
OXA-23AGGAATTCCATGAATAAATATTTTACTTGCblaOXA-23
OXA-23BCGGGATCCCGTTAAATAATATTCAGGTCblaOXA-23, reverse primer
OXA-40AGGAATTCCATGAAAAAATTTATACTTCCblaOXA-40
OXA-40BCGGGATCCCGTTAAATGATTCCAAGATTTTCTAGCGblaOXA-40, reverse primer
preOXA-58AGGAATTCCATGAAATTATTAAAATTGAGTTTAGblaOXA-58
preOXA-58BCGGGATCCCGTTATAAATAATGAAAAACACCCblaOXA-58, reverse primer
OXA-IMP1GCAAATAMAGAATATGTSCCblaOXA-40, internal primer
OXA-IMP2CTCMACCCARCCRGTCAACCblaOXA-40, internal reverse primer
M13 reverseGTCCTTTGTCGATACTpCR-BluntII-TOPO reverse sequencing primer
  • a M is A or C, S is G or C, and R is A or G.