TABLE 4.

Determination of mutation in QRDRs of gyrA and gyrB of MXF-resistant colonies grown from day 56 lung homogenates

Treatment group (% MXF in diet)aMouseGrown on plate containing indicated MXF concn (μg/ml)QRDR mutation, codon sequence change
gyrAgyrB
3 (0.125)12.0A90V, GCG→GTGNone
4 (0.25)1NAbNANA
20.5NoneNone
1.0D94G, GAC→GGCNone
2.0D94G, GAC→GGCNone
32.0D94G, GAC→GGCNone
2.0D94G, GAC→GGCNone
2.0D94G, GAC→GGCNone
2.0D94G, GAC→GGCNone
2.0D94G, GAC→GGCNone
41.0NoneNone
5 (0.5)12.0NoneNone
2.0D94G, GAC→GGCNone
22.0D94Y, GAC→TACNone
2.0D94Y, GAC→TACNone
2.0D94Y, GAC→TACNone
32.0NoneNone
2.0S91P, TCG→CCGNone
6 (1)12.0A90V, GCG→GTGNone
2.0D94N, GAC→AACNone
22.0NoneNone
  • a Treatment group 5 and 6 MXF-resistant colonies were grown from day 56 frozen lung homogenates.

  • b NA, not applicable.