TABLE 1.

Bacterial strains and plasmids used in this study

Strain or plasmidGenotype or characteristic(s)Source or reference
Strains
    EPEC E2348/69Prototypic O127:H6 EPEC strain8
    ΔescNescN deleted from E2348/6913
    ΔescCescC deleted from E2348/6913
    ΔescJescJ deleted from E2348/69N. Thomas, unpublished
    EAEC 17-2MotileT. Steiner
    EAEC 17-2NonmotileT. Steiner
Plasmids
    pescC-HSVescC with HSV on C-terminal portion cloned into tet gene (BamHI-SalI) of pACYC18413
    pKK232-8pBR322 derivative containing promoterless chloramphenicol acetyltransferase (cat) genePharmacia
    pLER-1179pKK232-8 derivative containing ler-cat transcriptional fusionV. H. Bustamante, unpublished
    pSEPZ-11pKK232-8 derivative containing LEE2-cat transcriptional fusion (pLEE2)1
    pSEPZ-17pKK232-8 derivative containing LEE2-cat transcriptional fusion (pLEE2-17)1
    pTIR394pKK232-8 derivative containing tir-cat transcriptional fusion37
    pLEE4-catpKK232-8 derivative containing LEE4-cat transcriptional fusionY. Martínez, unpublished
    pEspC-catpKK232-8 derivative containing espC-cat transcriptional fusionV. I. Martínez, unpublished
    pCAT232pKK232-8 derivative containing bfpA-cat transcriptional fusion36
    pKptsH-catpKK232-8 derivative containing ptsH-cat transcriptional fusionN. Olivares, unpublished
    pKaroG-catpKK232-8 derivative containing aroG-cat transcriptional fusionN. Olivares, unpublished
    pKpyrC-catpKK232-8 derivative containing pyrC-cat transcriptional fusionN. Olivares, unpublished