TABLE 1.

Primers used for sequencing of the blaSIM-1 gene and for detection of MBL genes

TargetPrimerUseSequence (5′ to 3′)PositionaReference
5′-CSINT1-FDetection and sequencingGGCATCCAAGCAGCAAG1256-127213
3′-CSINT2-RDetection and sequencingAAGCAGACTTGACCTGA4336-4620
intI1INT7-RSequencingGTTCTTCTACGGCAAGGTGC956-93712
arr-3ARR3-FSequencingGGTGACTTGCTAACCACAG91-10925
ARR3-RSequencingACAGTGACATAGCAAGTTCAG211-191
aadA1AADA1-FSequencingTGATTTGCTGGTTACGGTGAC144-16412
AADA1-RSequencingCACTACATTTCGCTCATCG561-543
blaIMP-1IMP1-FDetectionCATGGTTTGGTGGTTCTTGT601-62029
IMP1-RDetectionATAATTTGGCGGACTTTGGC1048-1029
blaVIM-2VIM2-FDetectionATGTTCAAACTTTTGAGTAAG1-2119
VIM2-RDetectionCTACTCAACGACTGAGCG801-784
blaSIM-1SIM1-FDetection and sequencingTACAAGGGATTCGGCATCG127-145This study
SIM1-RDetection and sequencingTAATGGCCTGTTCCCATGTG697-678
blaSIM-1SIM1-Exp/fCloning and expressionGGTCTAGAAGGAGAGTTAAAAATGAGAACTTTATTGATTT1-19This study
SIM1-Exp/rCloning and expressionCCGGATCCTTAATTAATGAGCGGCGGTTTTG719-741
16S rRNA8FSequencingAGAGTTTGATCCTGGCTCAG8-2715
1541RSequencingAAGGAGGTGATCCAGCCGCA1541-1522
  • a Position numbers correspond to the nucleotides of the coding sequences, except for the integron, for which position number 1 is the first nucleotide of the 5′ conserved segment (AY046276). For the SIM1-Exp primers, the regions annealing to the blaSIM-1 coding sequence are underlined.