TABLE 2.

MBL-producing Pseudomonas spp. and Acinetobacter spp. detected at a tertiary-care hospital in 2003-2004

Species (no. of isolates)No. of imipenem- resistant isolatesNo. (%) of isolates positive for:
MBL screeningablaIMP-1blaVIM-2blaSIM-1
P. aeruginosa (5,970)64434 (5)2 (6)32 (94)0
P. putida/P. fluorescens (211)45b42 (93)042 (100)0
Acinetobacter spp. (3,294)545135 (25)45 (33)83 (61)7 (6)
Total (9,475)1,234211 (17)47 (22)157 (74)7 (4)
  • a By the Hodge test and the imipenem and EDTA-SMA double-disk synergy test.

  • b Phenotypic differentiation of P. fluorescens and P. putida was performed for all imipenem-resistant isolates. The phenotypic identification of P. putida was confirmed by sequencing 16S rRNA from four randomly selected isolates.