TABLE 1.

Bacterial strains and plasmids used in these experiments

Bacterial strain or plasmidRelevant antimicrobial resistanceDescription
E. faecium strains
    C68Apr VmrClinical isolate initially used to define sequences downstream of pbp5 (2)
    D344RApr Vms, Eryr, TcrAmpicillin-resistant clinical isolate (21)
    D344SRFAps (pbp5 negative) Eryr Tcr, Fusr RifrPBP5, ampicillin-susceptible derivative of strain D344R (11)
    TX0016Low-level Apr, VmsAmpicillin-resistant clinical isolate whose partial genome sequence is available (Baylor College of Medicine [ftp://ftp.hgsc.bcm.tmc.edu/pub/data/Efaecium]; Joint Genome Institute [http://genome.jgi-psf.org/draft_microbes/entfa/entfa.home.html])
    D14Apr VmsAmpicillin-resistant clinical isolate originally isolated from Providence, R.I. (4), clonally distinct from strains D24, D25, and D29
    D24Apr VmsAmpicillin-resistant clinical isolate originally isolated from Richmond, Va. (4), clonally distinct from strains D14, D25, and D29
    D25Apr VmsAmpicillin-resistant clinical isolate originally isolated from Durham, N.C. (4), clonally distinct from strains D14, D24, and D29
    D29Apr VmsAmpicillin-resistant clinical isolate originally isolated from Calif. (4), clonally distinct from strains D14, D24, and D25
    TJ386Apr VmrAmpicillin- and vancomycin-resistant clinical isolate originally isolated from Philadelphia, Pa. (kindly provided by Henry Fraimow [18])
    WB312Apr VmrAmpicillin- and vancomycin-resistant clinical isolate originally isolated from Scranton, Pa. (kindly provided by Henry Fraimow [18])
    WC176Apr VmrAmpicillin- and vancomycin-resistant clinical isolate originally isolated in Westchester, N.Y. (kindly provided by Henry Fraimow [18])
    T420Low-level Apr, VmsStrain derived from turkey feces (did not yield transconjugants) (19)
    T450Low-level Apr, VmsStrain derived from turkey feces (did not yield transconjugants) (19)
    T471Low-level Apr, VmsStrain derived from turkey feces (did yield transconjugants) (19)
    T636Low-level Apr, VmsStrain derived from turkey feces (did yield transconjugants) (19)
    T642Low-level Apr, VmsStrain derived from turkey feces (did yield transconjugants) (19)
    GE-1Aps (pbp5 negative) Fusr RifrRecipient for enterococcal mating experiments, referenced here because it was the recipient strain used in the mating experiment that created CV133 (2)
    CV133Apr VmrAmpicillin- and vancomycin-resistant transconjugant resulting from mating between C68 and GE-1 (2); genomic DNA was the source for probe 4 as depicted in Fig. 1; in this region, it is identical to the genome of C68
Plasmids
    PBCSK(−)CmrCloning vector (Stratagene)
    pCWR570Cmr18-Kb PstI fragment of CV133 genomic DNA cloned into pBCSK−
    PCWR576Cmr5-Kb XbaI digestion fragment of insert from pCWR570 cloned into pBCSK−