TABLE 2.

Comparison between MICs of M. genitalium strains as determined by the cell culture method and conventional broth dilution test

StrainMIC (mg/liter)
DoxycyclineTetracyclineErythromycinLevofloxacin
Cell cultureaConventionalbCell cultureaConventionalbCell cultureaConventionalbCell cultureaConventionalb
InitialFinalInitialFinalInitialFinalInitialFinal
G370.50.12540.50.520.1250.0040.031224
M300.50.2540.250.12510.1250.0040.00420.52
M22820.250.0310.250.250.0310.0310.0630.0020.00210.50.5
M23000.250.2510.250.0310.250.1250.0040.00840.51
M23210.50.2540.50.520.0630.0020.02412
M23411244240.250.0630.06310.52
M60900.1250.2520.1250.2510.0630.0080.0210.51
M61510.250.2520.250.2520.1250.0040.02111
  • a TaqMan 5′ nuclease real-time PCR. The MIC value was defined as the lowest concentration of antibiotics causing 99% inhibition at 3 weeks after the incubation of M. genitalium with Vero cells.

  • b The MIC value was defined as the lowest concentration of antibiotics showing no change of color at the initial and final readings. The initial MIC was read when a change of color in the control wells was first observed, and the final MIC was read when no further color change could be observed.